Evaluation of Enzyme Immunoassay Technique (Hepanostika) for the Demonstration of Hepatitis B Surface Antigen
نویسندگان
چکیده
منابع مشابه
Solid-phase enzyme immunoassay for hepatitis B surface antigen.
A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially...
متن کاملSolid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.
The preliminary results of a solid-phase enzyme-immunoassay (EIA) for the detection of hepatitis B surface antigen (HBsAg) are presented. This method has been compared with the solid-phase radioimmunoassay (RIA) for HBsAg in dilution series of four HBsAg positive sera four national reference panels (The Laboratory Panel of the Central Laboratory of the Blood Transfusion Service of the Netherlan...
متن کاملDetection of human antibodies to hepatitis B surface antigen (HBsAg) by an enzyme-immunoassay for HBsAg.
We studied the feasibility of detecting antibody to hepatitis B surface antigen (anti-HBs) by an inhibition assay using the reagents of an enzyme-immunoassay for HBsAg (Hepanostika). Several modifications of the basic assay were investigated. Sensitivity was greatest when the test sample was incubated with a predetermined amount of HBsAg before the usual procedure of HBsAg detection. The presen...
متن کاملEXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...
متن کاملFalse-positive results with third-generation monoclonal hepatitis B surface antigen enzyme immunoassay.
Nonspecific hepatitis B surface antigen reactions with a third-generation enzyme immunoassay (Auszyme Monoclonal; Abbott Laboratories, North Chicago, Ill.) were investigated with 9,577 serum specimens in a clinical laboratory setting. Of the 196 serum specimens found reactive in Auszyme screen by the overnight procedure, 103 turned out to be true-positives, 71 were nonrepeatably reactive, and 2...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 1981
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.55.155